Infectolab-Vet is a small, specialised veterinary laboratory that was created in June 2014. The idea to further develop the veterinary diagnostics emerged from numerous requests from horse and household pets owners which are (or were) patients of the BCA-clinic in Augsburg.
Together, the BCA-clinic, its associated specialised laboratory Infectolab and the biotechnological corporation AIF-Diagnostika developed a new diagnostic method to detect a possible Borrelia infection in dogs and horses. In addition to the antibody tests, a procedure was developed that shows possible infections by measuring the cellular immune system.
It has been established that for many pets such as dogs the infection rate can reach up to 50% in certain European regions. Even for horses, the risk of infection is continuously rising. This risk increases on holiday trips to foreign countries, where pets are exposed to unfamiliar pathogens that they do not encounter in their home country. This also applies to riding, show and competition horses; in this instance, horseflies represent another method of infection.
The clinical symptoms for dogs and horses are similar to the human disease. Typical signs for dogs are an impaired general health, anorexia, fever, pain, swelling and lameness¹. In horses, we can observe eye diseases, chronic weight loss, sporadic lameness and shifting leg lameness, muscles pain, arthritis and other orthopedic impairments².
The most common tests for Borrelia infection in pets are ELISA and Westernblot. However, in collaboration with the biotechnological corporation AID-Diagnostika, an EliSpot has been developed specifically to create a trustworthy laboratory diagnostic for horses and dogs that may be infected with Borrelia.
As it is the first specialised laboratory, Infectolab can offer such test procedures. The EliSpot has been used in human medicine for many years, in order to assess the cellular immune response and its activity. The EliSpot is a standardised procedure that is externally quality-controlled and is mainly used and accredited for the diagnosis of tuberculosis. In cases of other pathogens (i.e. infections such as Borrelia), different antigens are used to achieve a stimulation of the T-Lymphocytes. In human medicine, this test is also known as the Interferon-Gamma-Test.
It has therefore become possible, thanks to the combination of those modern test procedures, to make conclusions on the humoral level (IgG-ELISA, Borrelia Blot) and on the cellular level (EliSpot) of the immune system. Those results, in correlation with the clinical evaluation, should allow a reliable statement to be made on whether the canine and equine Borrelia infection needs to be treated.
For more information on the EliSpot test procedures, please visit: www.elispot.com
Please note that as known from the experiences in the human medicine, the sensitivity of the Elispot tests is influenced by the administration of antibiotics or Cortisol. Therefore the last drug dispensation should at least be dated back 4 weeks, at a unique administration (no depot) at least 14 days.
Infectolab-Vet also offers the opportunity to check the ticks for possible agents (and human-pathogen strains in particular) by means of the PCR. This is especially interesting, not just for pet owners, but also for people who enjoy outdoor activities. Please visit Zecken-PCR.
¹: Reinhard K. Straubinger; PhD, Inke Krupka, Dr med vet: Lyme Borreliosis in Dogs and Cats: Background, Diagnosis, Treatment and Prevention of Infections with Borrelia burgdorferi sensu strict, Vet Clin Samall Anim 40 (2010) 1103-1119
²: Animal Health Diagnostic Center, Cornell University, College of Veterinary Medicine: Lyme Disease Multiplex Testing for Horses (version 2/2014)
You can choose between two laboratory test order forms::
To start with, please request your blood kit for your horse at:
+49 (0) 821 455 190 32
+49 (0) 821 455 074 1
On the laboratory test order, you will be able to recognise the tubes that correspond to each test.
Please note that the EliSpot imperatively requires CPDA-tubes (yellow cap). Please keep the blood at normal room temperature and do not centrifuge it. The blood must reach the laboratory within 72 hours, as the EliSpot requires ‘living’ cells. Please also be aware that all blood samples must arrive in Infectolab-Vet by Saturday at 10:00 a.m. for processing, as we are closed on Sundays.
Since we are a small laboratory, we ask you to consider the following logistical aspects:
Please send the blood samples inside the kit. You can send single kits using the service of the Deutsche Post (German Post Office) or any another courier service. For more than one kit, the laboratory will request a pickup service with GO!-Logistik. Please inform us via phone so that we can organise this for you. We recommend that you send all of the blood samples that are going to be analysed from all of the animals in one courier shipment.
The laboratory results are sent to the veterinarian and/or the owner. Should you require the results via fax, please let us know.
We use a pre-payment system for foreign clients. You will find an additional document in your kit for this purpose. Please transfer the total price for the tests and the additional fee onto our account. As soon as the payment has arrived, we will send over your laboratory results.
It is possible for several veterinarians to organise one collective invoice.
Please let us know if you are interested!
Please keep in mind that from experiences in human medicine the specificity of the EliSpot test is affected by antibiotics or by immunosuppressives (like cortisol). For this reason the last medication of these drugs should be back at least 4 weeks, unique medication (no deposit) at least 14 days.
Tick Testing (PCR) – Tick testing – to be completely sure!
As a specialised laboratory for tick-borne diseases, we can test any tick and guarantee rapid and reliable results. In addition to Borrelia, we also recommend testing for other important pathogens.
Therefore, we offer four different tests (PCR) in one package, including:
3. Babesia (important for dogs)
The laboratory test order forms can be downloaded here:
English in US $
Dead ticks can also be tested. Just stick the tick (or the parts of the tick) onto a piece of paper with adhesive tape. You can then send this paper, together with the completed and signed laboratory order, in an envelope to Infectolab-Vet.
Ten days after we receive the tick(s), we will provide you with a written result of the PCR test and an invoice. Please make the payment as soon as possible.
Please note that health insurance companies do not cover the costs for these tests.
Why should the tick be tested?
Even small ticks may be infected. A tick develops from an egg to a larva and then to a nymph (young tick). The nymph is a hermaphrodite (male and female), which can either develop into a female or male adult tick. Every nymph needs a blood meal. All developmental stages can transfer pathogens of infection.
It is commonly known that ticks can transmit dangerous infectious diseases. Only TBE (Tick-Borne-Encephalitis) is usually mentioned and there is an effective vaccine for TBE. The risks of Lyme disease and other co-infections that can also results from a tick bite are largely underestimated; there is no effective vaccine against these infections.
Approximately 1% of larvae, 10% of nymphs and 20% of adult ticks carry the pathogen borrelia burgdorferi sensu lato. A study has shown an incidence of 111/100.000 inhabitants per year in the area of Nürnberg. Consequently, there will be around 90,000 new infections in Germany every year. In southern Germany, 226 ticks have been analysed and it appeared that 21.2% were infected with B. burgdorferi sensu lato and another 3.5% carried babesia microti and other pathogens. More than one pathogen was found in over 7% of the analysed ticks.
Previously, isolated studies have been carried out to evaluate the infection rate of ticks with Borrelia and other co-infections. A Belgian study (V. Obsomer, PhD et al., Unvisersité Catholique de Louvian) detected up to 95 different pathogens in ticks. In certain studied areas, 45% of the ticks were currently infected with Borrelia. It is believed that 30% of ticks that are spread through birds carry up to six different human Borrelia stems.
Humans in the north-eastern and Midwestern United States are at increasing risk of acquiring tick-borne diseases; not only Lyme disease, but also two emerging diseases, human granulogcytic anaplasmosis and human babesiosis. Scientists found significant deviations in levels of co-infections in questing nymphs, most notably 83% more co-infection with Babesia microti and Borrelia burgdorferi than predicted by chance alone.
The proliferation of infected ticks depends on temporal, climatic and geographical changes. The living environment of different wild animals and the modified migration directions of birds is an important factor, given that all those animals are hosts for Borrelia and that they all carry ticks and participate in their spreading. Birds that live mainly in grass, shrubbery and underbrush and that spread infected ticks are particularly interesting. Ticks also survive in different new regions due to current climate changes. The human’s travelling behavior in our globalised era also favours the proliferation of infected ticks (means of transport, clothes, luggage, etc.) Furthermore, there are different types of ticks that are infected with different types of pathogens. Therefore the Borrelia stem borrelia miyamotoi, which has first occurred in the Pacific region in 1995, can now be observed in areas such as southern Germany, Italy, Holland, and in northern America.
29. October 2016, 14.00 to 17.00
Infotag der BCA-clinic Betriebs GmbH & Co. KG anlässlich ihres 10-jährigen Bestehens
Mehr Informationen finden Sie hier.
Literature & Studies
Animal Health Diagnostic Center, Cornell University, College of Veterinary Medicine: Lyme Disease Multiplex Testing for Horses (version 2/2014)
Balakrishnan N et al.: Serological and molecular prevalence of selected canine vector borne pathogens in blood donor candidates, clinically healthy volunteers, and stray dogs in North Carolina; Parasit Vectors. 2014 Mar 24; 7:116
Barth C. Et al.: Comparison of different diagnostic assays for the detection of Borrelia burgdorferi-specific antibodies in dogs; Vet Clin Pathol 0/0 (2014) 1-9
Marion Blaschitz et al.: Babesia Species Occurring in Austrian Ixodes ricinus Ticks; Applied and environmental Microbiology, Aug. 2008, Vol. 74, No. 15, p. 4841-4846
Peter D. Burbelo et al.: Antibody Profiling of Borrelia burgdorferi Infection in Horses; Clinical and Vaccine Immunology, Sept. 2011, p. 1562-1567
E.C. Burgess: Experimental inoculation of dogs with Borrelia burgdorferi; Zbl. Bakt. Hyg. A 263: 49 – 54, (1986)
Olaf Dietz, Bernhard Huskamp: Handbuch der Pferdepraxis, ISBN: 3-8304-1028-X 35.15 Borreliose von A. Liebisch
Thomas J. Divers: EQUine Lyme Diseases; Journal of Equine Veterinary Science 33 (2013) 488-492
Cyrille Bilé Ehounoud et al.: Multiple Pathogens Including Potential New Species in Tick Vectors on Côte d’Ivoire, 2016. PLoS Negl Trop Dis 10(1):e0004367.doi:10.1371/jounal.pntd.0004367.
Mark W. Eshoo et al.: Broad-Range Survey of Tick-Borne Pathogens in Southern Germany Reveals a High prevalence of Babesia microti and a Diversity of Other Tick-Borne Pathogens; Vector-Borne and Zoonotic Diseases, Volume 14, Number 8, 2014
Michelle H. Hersh et al.: Co-Infection of Blacklegged Ticks with Babesia microti and Borrelia burgdorferi Is Higher than Expected and Acquired from Small Mammal Hosts; PLOS ONE, June 2014, Volume 9, Issue 6, e99348
Honsberger Nicole A. et al.: Efficacy of sarolaner in the prevention of Borrelia burgdorferi and Anaplasma phagocytophilum transmission from infected Ixodes scapularis to dogs. Vet. Parasitol (2016), http://dx.doi.org/10.1016./j.vetpar.206.02.010
Paul M. Lantos et al.: Detection of Bartonella Species in the Blood of Veterinarians and Veterinary Technicians: A Newly Recognized Occupational Hazard?; VECTOR-BORNE AND ZOONOTIC DISEASES Volume 14, Number 8, 2014, DOI:10.1089/vbz.2013.1512
Erica Larson: Equine Lyme Neuroborreliosis: What We Know, What We Don’t; THE HORSE, Jul 08, 2014, Article 34157
Mc Cown ME et al.: Surveillance for Ehrlichia canis, Anaplasma phagocytophilum, Borrelia burgdorferi, and Dirofilaria immitis in Dogs From Three Cities in Columbia; J Spec Oper Med. 2014 Spring; 14 (1):86-90
Magnarelli: The etiologic agent of LD in Deer Flies, Horse Flies and Mosquitoes. J Inf Dis. 54(2) p.355 ff, 1986
Magnarelli: Ticks and Biting Insects with the Etiologic Agent of L. Dis. Borrelia Burgdorferi. J Clin Microbiol. 1482-86, 1988
Lorraine Michelet et al.: High-throughput screening of tick-borne pathogens in Europe; Fronliers in Celluar and Infection Microbiology, Juli 2014, Volume 4, Article 103
Moroff S et al.: Detection of antibodies against Anaplasma phagocytophilum in dogs using an automated fluorescence based system, Vet J. 2014 Aug 19. pii: S1090-0233(14)00339-6. doi: 10.1016/j.tvjl.2014.08.018. [Epub ahead of print]
Obsomer, V. et al.: Spatial disaggregation of tick occurence and ecology at a local scale as a preliminary step for spatial surveillance of tick born diseases: general framework and health implications in Belgium; in parasites&vectors, 22.6:190 Epub 2013, Jun. 22
Heather L. Priese et al.: Diagnosis of Borrelia-associated uveitis in two horses, Case report; Vererinary Ophahalmology (2012) 15, 6, 398-405
Susanne Schönert, A. Grabner: Lyme-Borreliose beim Pferd – aktueller Stand; PFERDE Vetkolleg
Susanne Schönert, Prof. Grabner: Untersuchungen zum direkten und indirekten Nachweis des Erregers der Lyme-Borreliose beim Pferd unter qualitätssichernden Aspekten; Dissertation Frei Uni Berlin 2004, http://www.diss.fu-berlin.de/diss/receive/FUDISS_thesis_000000002151
Gerold Stanek et al.: Lyme borreliosis; published online September 7, 2011 (www.thelancet.com, Vol379, Febr 4, 2012), DOI: 10.1016/SO140-6736(11)60103-7
Schramm F et al.: First detection of Borrelia burgdorferi sensu lato DNA in king penguins (Aptenodytes patagonicus halli); Ticks Tick Borne Dis. 2014 Oct;5(6):939-42. doi: 10.1016/j.ttbdis.2014.07.013. Epub 2014 Aug 20.
Scott John D et al.: Ticks parasitizing gallinaceous birds in Canada and first record of Borrelia burgdorferi-infected Ixodes pacificus (Acari: Ixodidae) from California Quail Systematic & Applied Acarology 21(1): 1–12 (2016); http://doi.org/10.11158/saa.21.1.1.
Reinhard K. Straubinger; PhD, Inke Krupka, Dr med vet: Lyme Borreliosis in Dogs and Cats: Background, Diagnosis, Treatment and Prevention of Infections with Borrelia burgdorferi sensu strict; Vet Clin Samall Anim 40 (2010) 1103-1119
Axel Teegler et al.: The relapsing fever Spirochete Borrelia miyamotoi resists complement-mediared killing human serum; Tick Tick-borne Dis (2014), http://dx.doi.org/10.1016/j.ttbdis.2014.07.011
Volgina NS et al.: Prevalence of borrelioses, anaplasmosis, chrlichiosis and Diroflaria immitis in dogs and vectors in Voronezh Reserve (Russia); Comp Immunol Microbiol Infect Dis. 2013 Dec; 36 (6): 567-74
Bettina Wagner et al.: Development of a multiplex assay for the detection of antibodies to Borrelia burgdorferi in horses and its validation using Bayesian and conventional statistical methods; Jounal: Veterinary Immunology and Immunopathology 144 (2011) 374-381
Wächter M et al.: Seroprevalence of spotted Fever group rickettsiae in dogs in Germany; Vector Borne Zoonotic Dis. 2015 Mar;15(3):191-4. doi: 10.1089/vbz.2014.1715
You can reach us:
Mon – Fri : 8 am – 5 pm
Your contact person for laboratory reports:
Infectolab-Vet Laborbetriebs GmbH
Dr. med. vet. Norbert Wolff, Veterinarian, specialised veterinarian for horses
Your contact person for all other questions:
+49 (0) 821 455 982 17
+49 (0) 821 455 982 15
Infectolab-Vet Laborbetriebs GmbH
Geschäftsführer: Dr. med. vet. Norbert Wolff
+49 (0) 821 455 982 17
+49 (0) 821 455 982 15
Tax ID: 103/129/32445
Registry court: District Court, Augsburg HRB Nr. 29779
1. Liability Limitation
The content of this website was created with the greatest possible caution. The provider however accepts no liability for the accuracy, completeness and currency of the content provided. The use of this website proceeds at risk to the user. Articles that are identified by name reflect the opinion of the respective author and not always the opinion of the provider. The mere use of the website does not establish any sort of contractual relationship between the user and the provider.
2. External Links
This website contains links to third party websites (“external links”). These websites are subject to the liability of their respective providers. When the links were first being set, the provider check the external content of the external links for any violations of the law. At that time no illegal content could be detected. The provider has however no influence of any kind on the current and future constitution of the content of the linked sites. Setting external links does not mean that the provider approves of the content behind the link. It is not reasonable to expect the provider to constantly monitor external links without concrete indications of violations of the law. In the event that illegal content is detected, however, such external links will be immediately deleted.
3. Copyrights and Ancillary Copyrights
The content published on this website is subject to German copyright and ancillary copyright law. Any user that is not permitted by German copyright and ancillary copyright requires the prior written consent of the provider or the respective rights holder. This applies especially to reproduction, editing, translation, electronic storage, processing, or representation of the content in databases or in other electronic media and systems. Content and rights of third persons are identified as such. The unauthorised reproduction or sharing of specific content or entire pages is not permitted and is punishable under law. Only the creation of copies and downloads for personal, private and non-commercial purposes is allowed.
The representation of this website in external frames is only allowed on condition of prior written consent.
4. Data Protection
When one visits the provider’s website, information about the site visit (date, time, page viewed) can be saved. These data are not personal data but are rendered anonymous. They are evaluated exclusively for statistical purposes. The data are not shared with third parties for commercial or non-commercial purposes.
The provider expressly points out that data transfer in the internet (e.g. e-mail communications) can evince security gaps and cannot be perfectly protected from third-party access.
The use of the contact data supplied in the publisher’s imprint for commercial advertisement is expressly prohibited unless the provider has issued prior written consent or there is already and existing business relationship. The provider and all persons mentioned on this website herewith deny permission to any commercial use and sharing of their data.
You can visit our website even without giving any personal data. To the extent that personal data (such as name, address or e-mail address) is collected on our pages, this is done on a voluntary basis to the extent possible. These data are not shared with third parties without your expressed permission. To the extent that a contractual relationship between you and us is to be established, expanded in content or modified or your are submitting a query to us, we collect and use personal data from you that is required for these purposes (inventory data). We collect, process and use personal data to the extent necessary in order to allow you to make use of our web sales (use data). All personal data are only saved as long as is required for the purpose specified (processing your query or concluding a contract). The mandatory retention periods under tax law and commercial law are taken into account. On request form the responsible agencies we can in individual cases release information about these data (inventory data) to the extent that this is necessary for purposes of criminal prosecution, to avert pending dangers, to fulfil legal requirements from the Office for the Protection of the Constitution or the Military Counterintelligence Service or to assert rights to intellectual property.
We collect personal data in the framework of the comment function (e.g. name, e-mail) in the framework of your comments on an article and only in the scope in which you communicate them to us. On publishing a comment, the e-mail address that you provide will be saved but not published. Your name will be published if you did not write under a pseudonym.
Data Confidentiality Disclaimer for the Facebook-Plug-in (“Like”)
This website uses plug-ins from the provider Facebook.com, which is operated by the company Facebook Inc., 1601 S. California Avenue, Palo Alto, CA 94304 in the USA. Users of our website on which the Facebook-plug-in (“Like”-button) is installed are herewith informed that the plug-in generates a connection with Facebook which then executes a communication to your browser so that the plug-in appears on the website.
Furthermore, by using it, you are forwarding data containing information about your visit to our homepage to the Facebook-server. For logged in Facebook-users this results in the use data being assigned to your personal Facebook-account.
As soon as you actively use the Facebook-plug-in as a logged in Facebook-user (e.g. by clicking on the “Like” button or using the comment function), these data will be transferred to you Facebook account and published. You can only avoid this by logging out of your Facebook account first.
You can obtain additional information about the use of your data by Facebook from Facebook’s own data confidentiality policy found at http://de-de.facebook.com/policy.php.
Data Confidentiality Disclaimer for the Social Network Google Plus
This website uses the so-called “G +1”-button for the social network Google Plus, which is operated by Google Inc., 1600 Amphitheatre Parkway, Mountain View, CA 94043, United States (“Google”). The button can be recognised by the symbol “G +1”. If you are registered with Google Plus, you can express your interest in our website with the “G +1” button and share content from our website on Google Plus. In that case Google saves both the information that you have given a “G+1” for our content “G +1” as well as the information about the page that you have viewed. Your “G +1” can be added optionally together with your name (possibly with your photo –to the extent present) into Google Plus and into other Google-services such as Google Search or your Google-Profile.
Data Confidentiality Disclaimer for the Use of the twitter.com Web Messaging Service
We have also integrated the web message service twitter.com on our website. It is operated by Twitter Inc., 1355 Market St, Suite 900, San Francisco, CA 94103, USA. Twitter offers the so-called “Tweet” – function. One can use to publish 140 character messages including website links in one’s own Twitter account. If you use Twitter’s “Tweet”-Function on our webpages, the specific website will be linked to your account on Twitter and published there. Data are transferred to Twitter.
We are not informed of the content of the transmitted data and their use by Twitter. Consult the Data Confidentiality Disclaimer of Twitter for additional information: http://twitter.com/privacy
Twitter gives you the option to set your privacy settings under the following link: http://twitter.com/account/settings.
Right to Disclosure
You have the right at any time to request disclosure of the data collected about your person free of charge and without compensation. You have the right at any time to revoke your consent to use the personal data you have given for future. To have this information disclosed contact the provider under the contact data given in the publisher’s imprint.
Source: Sample-disclaimer from Juraforum.de in cooperation with bista.de online-law consulting.
© Copyright 2016